Journal of Animal Research and Nutrition

Comparative Study between Three-Step In Vitro and Modified Three-Step In Vitro Procedures in Small Intestinal Digestibility of Rumen Undegraded Protein

The experiment was carried out using fistulated multiparous Chinese Holstein dairy cows in their lactation period to evaluate the nutritive value of local protein feed resources. Rumen Undegraded Protein (RUP) digestibility in eleven types of concentrates was estimated by Three Step in vitro Procedure (TSP) and Modified Three Step in vitro Procedure (MTSP) and correlation. There were eleven experimental feeds were chosen and air-dried to investigate the effect of different growth period and variety on nutrition value and its RUP digestibility.In this experiment, the small intestinal digestibility of RUP by TSP in concentrated feed was about 65%, higher than in roughages, among them, SBM (soybean mean) had the highest percentage (79%) and corn had the lowest (65%), DDGS (distillers dried grains with solubles) and SFM (Sunflower Meal) were 70.9 and 74.9%, respectively. ASS (alfalfa mowed at squaring stage) had the highest small intestinal digestibility of RUP (55%) among roughages, and WCS (Whole-Plant Corn Silage) had the lowest (40.5%).

When small intestinal digestibility of RUP was determined using the MTSP method, exhibited similar results to the TSP method but values were generally higher, and there was a strong significant correlation between them (R₂=0.967, p< 0.01).

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