Abstract

Low Grade Inflammation in Visceral and Subcutaneous Adipose Tissue Originated from Adipose Derived Stem Cells in Experimental Model of Obesity in Rats under Influence of Pulsed Electromagnetic Field Interaction

Objective: Aim of our study was to examine if pulsed electromagnetic field (PEMF) treatment of the adipose derived stem cells (ADSCs) originating from differently localized adipose tissue, affects the pro-inflammatory cytokines and resistin release dependently on age, gender and used diet.

Methods: Cultures of ADSCs originated from pups and adult animals of both gender grown on low fat ( LF) and high fat (HF) diet for 21 days of obesity inducing time, after which the adipose tissue and blood samples for serum were collected. ADSCs cultures were exposed to PEMF (7 Hz, 30 mT) thrice, 4 h per day. Proinflammatory cytokines and resistin levels were determined by ELISA.

Results: The serum level of TNFα and IL-6 cytokines in HF diet female and male adults was significantly higher as compared to LF diet serum level. HF diet also caused changes in resistin serum level. ADSCs of PEMF exposed LF diet female pups released more TNFα than unexposed ones. IL-6 level in ADSCs cultures of PEMF exposed female pups was higher than in controls. PEMF exposure of ADSCs of both genders of HF diet pups caused increased secretion of TNFα. On the other hand IL-6 release by ADSCs of female pups upon PEMF treatment was diminished. PEMF exposure of ADSCs of both genders of LF diet pups caused increased secretion of resistin. Different results were obtained in case of HF diet pups ADSCs. ADSCs cultures after PEMF exposure originating from male and female adults produced more resistin.

Conclusion: We found that PEMF exposure can modify metabolic activity of ADSCs.


Author(s):

Baranowska Agnieszka, Skowron Beata, Ciesielczyk Katarzyna, Guzdek Piotr, Gil Krzysztof, Kaszuba-Zwoińska Jolanta



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