Journal of Animal Research and Nutrition

Effect of Vitamin C and D on Growth and Immunology of Rohu La ( beo Rohita) Fingerlings in District Lahore

The present study was scheduled for 90 days to determine the effect of vitamin C and D on growth and immunology of Rohu (Labeo rohita) fingerlings at Fisheries Research and Training Institute, Manawan district Lahore, Punjab, Pakistan in 12 different ponds (July 1 to September 30, 2019) with 3 different treatments and a control one. Four levels of treatment were used vitamin C (0.00, 0.23 g, 0.48 g, and 0.69 g) and vitamin D (0.00, 0.26 g, 0.43 g, and0.68 g) in triplicate groups. Supplementary feed that was composed of sunflower meal, maize gluten meal and rice polish (1:1:1) was applied to fish @ 2% of fish body weight twice a day. Artificial diets were formulated which contained 32.20% crude protein. All the water quality assessments/parameters fluctuated in values during the course of an experiment like temperature ℃, dissolved oxygen, pH, carbon dioxide, alkalinity, total hardness and planktonic biomass. All these parameters were tested on every 10^th day so each pond was sampled thrice in a month. Final weight was (wt) 265.4 ± 4.15 g (control), 272.5 ± 2.42 g (T₁), 280.5 ± 2.80 g (T₂) and 291.0 ± 5.75 g (T₃) respectively. SGRs were 0.6 ± 0.01% (control), 0.6 ± 0.01% (T₁), 0.8 ± 0.01% (T₂) and 0.5 ± 0.03% (T₃) respectively. Weight gain was 18.6 ± 0.75 g (control), 20.0 ± 0.47 g (T₁), 24.8 ± 2.25 g (T₂) and 23.4 ± 1.68 g (T₃) respectively. Survival rate (%) was 56.6 ± 2.51% (control), 60.3 ± 3.51% (T₁), 85.3 ±2.51% (T₂) and 72.3 ± 1.52% (T₃) respectively. Average daily weight gain was (ADG) 0.2 ± 0.01 (control), 0.2 ± 0.01 (T₁), 0.2 ± 0.01 (T₂) and 0.2 ± 0.01 (T₃) respectively which showed better results than other treatment groups. The vitamin C (AAs) and vitamin D was resolved in blood erythrocyte (1.5 ± 0.06 (T₁), 1.5 ± 0.08 (T₂), 2.3 ± 0.06 (T₃) and 1.8 ± 0.12 (T4) respectively, hemoglobin 4.6 ± 0.30 (T₁), 5.9 ± 0.73 (T₂), 7.8 ± 0.25 (T₃) and 7.1 ± 0.05 (T4) respectively). Body flesh enhanced the growth and immune system of fish. In control group (without vitamin C and D diet) hemoglobin levels decreased which might have decreased the oxygen carrying capacity in fish. Increased bacterial infection was perceived in the group which was fed on supplemented diet without vitamin C and D. Supplementary diet T₂ indicated significantly (p<0.05) higher growth than the other treatments and control one. In Pakistan, supplementary diets of vitamin C and D were affected on the immunological parameters and growth assessment of fish Rohu (Labeo rohita).

Author(s): Aamira Urooj, Muhammad Ashraf, Aasma Nourin, Masroor Elahi Babar, M Tariq Pervaiz, and Tanveer

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