Journal of Animal Research and Nutrition

In vitro, evaluation of aflatoxin B1 binding by strains of probiotic lactic acid bacteria, Lactobacillus reuteri DSM 20016T and Pediococcus pentosacus DSM 20206, isolated from chicken gut

The potential of Lactic Acid Bacteria (LAB) strains to remove Aflatoxin B1 (AFB1) has been reported in previous studies. The present study evaluated the binding capabilities of Lactobacillus reuteri and Pediococcus pentosacus, which were isolated from the chicken gut. To assess the AFB1 binding abilities of these LAB strains, an in vitro experiment was conducted. The LAB strains were incubated with AFB1 (5 μg/mL) at a temperature of +37°C for one hour. After centrifugation, the supernatant fluids were analyzed using High-Performance Liquid Chromatography (HPLC) to quantify any unbound AFB1 remaining in the solution. The results of this in vitro experiment indicated that Lactobacillus reuteri DSM 20016T exhibited a binding rate of 55.28% for AFB1, while Pediococcus pentosacus DSM 20206 showed a binding rate of 36.24% in the supernatant. These findings highlight the potential of these LAB strains as probiotic candidates with AFB1 binding properties; further research and validation are needed to confirm their effectiveness in vivo.

Author(s): Kasech Melese*, Tesfaye Alemu, Asnake Desalegn, Bekisisa Urge and Abebe Olanne

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